nicking response catalysed via the MobA89K and MobC89K proteins. The cleaved strand is then unwound because of the helicase and sent to the 89K-encoded GI-type T4SS equipment. The VirB1-89K part is believed to act as a lytic transglycosylase, regionally opening the peptidoglycan with contributions from A few other factor(s) during the assembly with